The Regiment was authorized on June 21, 1776 in the Continental Army as "Dubois' Regiment." The regiment was assigned to the Northern Department. Partially organized in the fall of 1776 in Albany. The regiment was made up primarily from veterans who served in Canada during operations in 1775-76. It was assigned on November 4, 1777 to the New York Brigade, an element of the Northern Department. New York Brigade relieved on May 24, 1779 from the Northern Department and assigned to the Main Continental Army. Reorganized on May 30, 1779 to consist of 9 companies. New York Brigade relieved on November 6, 1780 from the Highlands Department and assigned to the Northern Department. Consolidated on January 1, 1781 with the 1st New York Regiment.