The Pasquotank County Regiment was one of the 35 existing county militias to be authorized as a regiment of the North Carolina militia by the North Carolina Provincial Congress on September 9, 1775. On December 22, 1775, the regiment was split into a 1st Pasquotank County Regiment and a 2nd Pasquotank Regiment. On May 9, 1777, the 2nd Pasquotank Regiment was renamed the Camden County Regiment and the 1st Pasquotank County Regiment reverted to just the Pasquotank County Regiment. It was active until the end of the war.