The Regiment was authorized on September 1, 1775 in the Continental Army as the 2nd North Carolina Regiment. Reorganized on January 4, 1776 to consist of eight companies. It was assigned on February 27, 1776 to the Southern Department. It was assigned on July 8, 1777 to the North Carolina Brigade, an element of the Main Continental Army. Reorganized on June 1, 1778 to consist of 9 companies. Captured on May 12, 1780 at Charleston, South Carolina, by the British Army. Reorganized in summer of 1781 at Hillborough and Salisbury to consist of nine companies and assigned to the North Carolina Brigade, an element of the Southern Department. Furloughed on January 1, 1783 at James Island, South Carolina. Disbanded on November 15, 1783.