Organized on February 28, 1776 at Alexandria and Dunfies to consist of 10 companies from Price William, Fauquier, Stafford, Louisa, Fairfax, King George, Loundon and Culpepper Counties. Reorganized on January 11, 1776 to consist of 10 companies by raising two more musket companies. It was assigned on February 27, 1776 to the Southern Department. Relieved on July 22, 1778 from the 3rd Virginia Brigade and assigned to the 2nd Virginia Brigade, an element of the Main Continental Army. Consolidated on May 12, 1779 with the 5th Virginia Regiment and redesignated as the 3rd Virginia Regiment, to consist of 9 companies. Relieved on December 4, 1779 from the 2nd Virginia Brigade and assigned to the Southern Department. Captured on May 12, 1780 by the British Army at Charleston, South Carolina. Disbanded on January 1, 1783.